中国美利奴绵羊磷脂酶C zeta基因真核表达载体的构建及其表达研究

doi:10.11843/j.issn.0366-6964.2014.04.005

Abstract

Abstract:

This experiment aimed to construct the eukaryotic expression vector of PLCζ，a preliminary study of its expression properties in progenitor cell line was conducted.PLCζ was amplified by PCR and cloned into vector pEGFP-N1，after correct identification，the recombinant plasmid pEGFP-N1-PLCζ was transfected to 293T cells and sheep fibroblasts.The expression and distribution of cell recombinant plasmid was observed under an inverted fluorescence microscope and laser scanning confocal microscope.After digestion and sequencing,the pEGFP-N1-PLCζ eukaryotic expression vector was successfully constructed，and its successful fusion expression with the green fluorescent protein was observed.This experiment realized the expression of pEGFP-N1-PLCζ in sheep fetal fibroblast cells，which will help us to study the activation of recombinant protein on the oocyte through nuclear transfer furtherly.

CLC Number:

S826
S813.3

LIU Qiang，HE Zhi-rui，WANG Yin-long，WU RE lihazi，SAI Wujiafu. Construction Eukaryotic Expression Vector and Its Expression of PLCζ Gene from Chinese Merino Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 541-546.

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Construction Eukaryotic Expression Vector and Its Expression of PLCζ Gene from Chinese Merino Sheep

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